The laboratories of the Institute of Liver Studies at King’s College London are well established for both basic and clinical research activities. The Paediatric Liver Centre at King’s College Hospital is the largest supra-regional paediatric hepatology/liver transplant service in the UK and one of the largest in the world. The Cell Isolation Unit at King’s College Hospital is a purpose-built GMP facility where cells are isolated from adult donor tissue under sterile conditions for clinical administration. It is licensed by the Human Tissue Authority (HTA) to process and store cells for human use. The laboratory routinely prepares cells for hepatocyte transplantation in paediatric liver patients but also for associated laboratory research. Because of its close proximity with the liver surgery/transplant services, the research laboratory also receives a good supply of liver segments from resections and split liver transplantations, yielding high quality primary hepatocytes, specifically banked for research use.
Partner 5 team leaders:
Dr Céline Filippi
Institute of Liver Studies
Dr Robin Hughes
Institute of Liver Studies
Main tasks in the project:
- Hepatocyte isolation, banking and provision to consortium partners for genotyping
- Hepatocyte phenotyping
For the past 15 years, C. Filippi has been involved in primary hepatocyte isolation and the study of cell metabolism and bioenergetics, in a variety of projects in the ‘Laboratoire de Bioenergetique Fondamentale et Appliquee’ (Basic and Applied Bioenergetic lab) in Grenoble, then at the University of Edinburgh (UK) for a project aimed at improving the metabolism of hepatic cell lines for a bioartificial liver support system. She also led a study aiming at producing and phenotyping a cell culture model of non-alcoholic fatty liver disease. She then focused on the mitochondrial biogenesis and function in human embryonic stem cells and derived hepatocyte-like cells in the MRC-Centre for Regenerative Medicine, Edinburgh and at Geron Corp (CA, USA). Her involvement in the Institute of Liver Studies at KCL adds to the expertise of the team led by Prof Dhawan and Dr Hughes who focuses in the refinement of the isolation and cryopreservation of adult human primary hepatocytes used for transplantation in children with inborn errors of liver metabolism.
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